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Kinesiology
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Molecular Exercise Physiology Laboratory

PI: Eunhee Chung, Ph.D.

Eunhee Chung

Lab Members

Lab manager – Yessenia Perez, BSc
Lab research scientist
– U-Ter Aondo Jia, MEng
Current Undergraduate Students: Juan Estevez III and Ayyan Mahboob
Lab Alumni: Justin Cruger (M.S. graduated in August 2017), Salvatore Campise (B.S. graduated in December 2017) and Jake Sansom (B.S. graduated in December 2018). Andrea Chinas Melvin (summer research student from Monterrey Institute of Technology, Mexico), Kassandra Gonzalez (MS, Summer 2020), Antonio Bollinger (BS), Jacqueline Gonzalez (BS), Erin Roye (BS), Christina Perez (BS), Kimberli Garcia (BS), and Jake Lota.

 

Research Interests:

My research has focused on two agendas: First, we investigate the impact of maternal diet and exercise on the cardiometabolic health of offspring. We wish to probe the mechanisms by which maternal exercise before and during pregnancy is a modifiable maternal behavior that may reduce the development of obesity-linked cardiometabolic dysfunction in offspring. Second, we have studied diet-induced obese mouse models to better understand the role of nutritional supplementation in type 2 diabetes, particularly skeletal muscle adaptation. An interdisciplinary approach, including functional measurement (echocardiography, pressure-volume hemodynamics, and myocyte contractile properties), mitochondrial function, gene expression, biochemical assays, immunohistochemistry, and histology has been used to investigate these topics.

Publications:

https://www.ncbi.nlm.nih.gov/sites/myncbi/18Og94g7_mpQd/bibliography/51981910/public/?sort=date&direction=ascending

Major Laboratory Equipment:

Echocardiography- in vivo cardiac function measurement

Echocardiographic measurement is performed under light anesthesia to measure cardiac function

 

 

 

 

 

 

 

O2K-FluRespirometry: Mitochondrial functional measurement

Mechanical preparation of cardiac fibers to measure mitochondrial function. Cardiac tissue is separated into small bundles ( 1mm diameter and about 2 mm length strip) to prepare saponin-skinned fibers

 

 

 

 

 

 

 

 

 

 

Mitochondrial function measurement using O2k-FluoRespirometry

 

 

 

 

 

 

Real-time quantitative PCR machine to measure gene expression

 

 

 

 

 

Chemiluminescence detection system to obtain an image

Cryostat and A fluorescent microscope: Immunofluorescence Analysis

Frozen muscle sections are cut into 8mm thick cryosection to characterize the skeletal muscle

 

 

 

 

 

 

 

Antibody treatment to the cryosection – primary antibodies were added to the muscle section to visualize the muscle fiber

 

 

 

 

 

 

 

 

Different colors indicate different muscle fiber (Blue- Type I, Green- Type IIa, Red- Type IIb, Unstained- Type IIx)

Department of Kinesiology

Phone: 210-458-6023
Fax: 210-458-5873
Location: MB 3.324
The University of Texas at San Antonio
One UTSA Circle
San Antonio, TX 78249-1644